Additional assessment of the expression of selected genes identified as differentially expressed between Phf6 deleted and control samples by RNA-sequencing.

<p>(A) RT-qPCR of <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> vs. <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> E15.5 cortex. As in the RNA-sequencing dataset, mRNA levels for the selected genes were higher in the <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> vs. <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> cortex. (B) Representative images of RNA/RNA whole mount in situ hybridisation of E15.5 <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> vs. <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> brains using RNA probes as indicated. N = 5–7 tissue samples per genotype. Data are presented as mean ± sem (A). Data from individual foetuses are represented as open circles (A). Data were analysed by two-way ANOVA (A).</p> <p>(PDF)</p>