Apoptotic cells play a role in p-ERK activity.

<p>(A) Representative images of p-ERK staining before and after MEK inhibitor (U0126) administration. (B) Normalized fluorescent intensities of p-ERK with and without MEK inhibitor. Two–three ROIs per tail were selected. <i>n</i> = 77, DMSO; <i>n</i> = 80, MEK inhibitor; <i>n</i> = 83, MTZ; <i>n</i> = MTZ + MEK inhibitor. **** <0.0001 one-way ANOVA with a Tukey’s ad hoc test. (C) Representative images of BCL-2 stains before and after heatshock. (D) The number of apoptotic cells after heatshock. <i>n</i> = 15, -HS, DMSO; <i>n</i> = 16, +HS, DMSO; <i>n</i> = 13, -HS, MTZ; <i>n</i> = 16, +HS, MTZ. **0.001, ****<0.0001. (E) Representative images of p-ERK stains in <i>Et(Gal4-VP16)</i><sup><i>zc1036A</i></sup>,<i>Tg(UAS-E1b</i>:<i>nsfB-mCherry)</i><sup><i>c264</i></sup>;<i>Tg(hsp70l</i>:<i>bcl2-2A-CAAX-GFP)</i> larvae under conditions of damage and heatshock. (F) p-ERK fluorescent intensity with and without heatshock. <i>n</i> = 45, -HS, DMSO; <i>n</i> = 27, +HS, DMSO; <i>n</i> = 33, -HS, MTZ; <i>n</i> = 31, +HS, MTZ. **0.001, ****<0.0001. Statistics for C and D were determined using a one-way ANOVA with a Tukey’s ad hoc test. The underlying data for the graphs in this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002194#pbio.3002194.s017" target="_blank">S2 Data</a>.</p> <p>(PDF)</p>