Gating strategy for sorting cells into different immune cells.

Initial gating was performed with forward (FSC) and side (SSC) scatters to isolate lymphocytes (PBMC). Further gating with FSC-A and FSC-W was done to exclude doublets. Live cells were selected based on the gating with DAPI. The live cells were gated on CD3 to separate between CD3+ and CD3- cells. The CD3+ population was further gated for CD4 and CD8, whilst the CD3- population was gated for CD14 and CD19. (PDF)

Publication Details

Journal
Figshare
Published
2025-03-07
DOI
https://doi.org/10.1371/journal.pcbi.1012859.s011
Primary Topic
Education Methods and Technologies
Type
article
Field-Weighted Citation Impact
0.00
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article

Gating strategy for sorting cells into different immune cells.

Figshare
Education Methods and Technologies
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Gating strategy for sorting cells into different immune cells.

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Abstract

Initial gating was performed with forward (FSC) and side (SSC) scatters to isolate lymphocytes (PBMC). Further gating with FSC-A and FSC-W was done to exclude doublets. Live cells were selected based on the gating with DAPI. The live cells were gated on CD3 to separate between CD3+ and CD3- cells. The CD3+ population was further gated for CD4 and CD8, whilst the CD3- population was gated for CD14 and CD19. (PDF)

Figshare
Openalex Percentile: Top 48%
Education Methods and Technologies
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Gating strategy for sorting cells into different immune cells. · Figshare (2025) | TGRS Research Map | TGRS