Detailed examination of DGE recovery measures calling significance at FDR < 0.05 in both imputed and observed data.

(A) Distributions of sensitivity and specificity of DGE recovery by cell type and approach. Each point is a simulated phenotype. (B) R-squared (Rsq, y-axis) and (C) slopes of imputed log2 fold changes (FC) regression on observed effect sizes by approach. Each point is a simulated phenotype, coloured by cell type. CIBX-inbuilt: CIBERSORTx with the inbuilt signature matrix; CIBX-custom: CIBERSORTx with a custom signature matrix derived; bMIND: bMIND with flow fractions; swCAM: swCAM with flow fractions; LASSO/ridge: regularised multi-response Gaussian models. (PDF)