Rgf1 cooperates with Mod5 in Tea4 anchoring to the cellular poles.

<p>(A) Representative images of the indicated strains producing Tea1<i>-</i>GFP. The maximum-intensity projection of 6 Z-slides (0.5 μm step-size) is shown. Scale bar, 2 μm. The graph represents the mean ± SD of the relative fluorescence intensity of Tea1-GFP measured at the cell tips in the WT, <i>rgf1</i>Δ, <i>mod5</i>Δ, and <i>rgf1</i>Δ <i>mod5</i>Δ cells (<i>n</i> > 100). WT levels were used for normalization. (B) Cell morphology of the indicated strains after refeeding treatment (-MBC). (C) Cell morphology of the indicated strains after 4 h at 36°C in YES liquid medium. Scale bar, 2 μm. ****<i>P</i> < 0.0001. The data underlying the graphs shown in the figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002491#pbio.3002491.s014" target="_blank">S1 Data</a>.</p> <p>(PDF)</p>