Serological and genetic analysis of a Bel subtype caused by a novel 29insG mutation: a case report

Objective To investigate the serological characteristics and molecular mechanism of a case of Bel subtype. Methods Serological method was used to analyze the ABO blood group of the patient, absorption and elution test was used to detect the weak antigen on red blood cells, and molecular biology method was used to analyze the ABO gene sequence of the patient. Results The forward typing of the patient was O type, but the reverse typing was B type. The weak B antigen was confirmed by absorption and elution method. This mutation was not found in ISBT and related literature, and was discovered for the first time. The patient's blood type was Bel (29insertG)/O.01.01 based on the sequence of exon 3-7. Conclusion In the case of ABO blood group incompatibility, the combination of serological and molecular biological detection techniques can improve the accuracy and reliability of ABO blood group identification.