Plasmids from the same PTU display different conjugation dynamics [Dataset]

(A) Conjugation kinetics for different plasmids of the same PTU. The conjugation frequency, expressed as Tc/ D is indicated on the y axis. The conjugation time, in minutes, is indicated on the x axis. (B) Circles indicating conjugation frequencies (y axis) obtained at different cell densities (x axis) for different plasmids belonging to PTU-W. Black lines indicate the interpolated [T]/[D] values according to Eq.3. Grey areas indicate the predicted confidence interval at 95% for the adjusted Kon and τ values. The estimated engagement times (τ) and encounter rates (kon), with their respective 95% confidence intervals are as follows: pMBUI4 Kon = 274 [131–580] μm2, τ = 4 [3.2–5.1]´; R388 Kon = 76 [28–263] μm2, τ = 18 [15–24]´; pIE321 Kon = 10 [4–32] μm2, τ = 36 [28–51]´; R7K Kon = 1 [0.3–4] μm2, τ = 262 [185–364]´.

Publication Details

Journal
DIGITAL.CSIC (Spanish National Research Council (CSIC))
Published
2025-02-07
DOI
https://doi.org/10.1371/journal.pgen.1011560.g004
Primary Topic
Radiation Shielding Materials Analysis
Type
article
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article

Plasmids from the same PTU display different conjugation dynamics [Dataset]

DIGITAL.CSIC (Spanish National Research Council (CSIC))
Radiation Shielding Materials Analysis
article

Plasmids from the same PTU display different conjugation dynamics [Dataset]

article en

Abstract

(A) Conjugation kinetics for different plasmids of the same PTU. The conjugation frequency, expressed as Tc/ D is indicated on the y axis. The conjugation time, in minutes, is indicated on the x axis. (B) Circles indicating conjugation frequencies (y axis) obtained at different cell densities (x axis) for different plasmids belonging to PTU-W. Black lines indicate the interpolated [T]/[D] values according to Eq.3. Grey areas indicate the predicted confidence interval at 95% for the adjusted Kon and τ values. The estimated engagement times (τ) and encounter rates (kon), with their respective 95% confidence intervals are as follows: pMBUI4 Kon = 274 [131–580] μm2, τ = 4 [3.2–5.1]´; R388 Kon = 76 [28–263] μm2, τ = 18 [15–24]´; pIE321 Kon = 10 [4–32] μm2, τ = 36 [28–51]´; R7K Kon = 1 [0.3–4] μm2, τ = 262 [185–364]´.

DIGITAL.CSIC (Spanish National Research Council (CSIC))
Openalex Percentile: Top 63%
Radiation Shielding Materials Analysis
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