Neurite length and number of cultured cortical neurons are unaffected by loss of PHF6.

<p>(A) Representative images from E16.5 <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> vs. <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> cultured cortical neurons showing staining with anti-beta III tubulin and DAPI. Scale bar = 21.25 μm. (B) Enumerations of the numbers of primary, secondary and tertiary neurites per neuron in control and <i>Phf6</i>-deleted cultured cortical neurons. No significant difference was detected. (C) Quantification of the length of primary, secondary and tertiary neurites in <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> vs. <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> cultured cortical neurons. No significant difference was detected. N = 4 <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> vs. 4 <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> foetuses. Data are displayed mean ± sem. Circles represent individual mouse foetuses. Data were analysed by using a two-tailed Student’s t-test.</p> <p>(PDF)</p>