<i>In vitro</i> bradyzoite differentiation of <i>T. gondii</i> at 33°C.

(A) Fluorescent imaging of HFF cells infected with ME49 mCherry for 8-days post-infection in HFF media or differentiation media. (B) Fluorescent images of DBA-, BAG1-, SAG1-positive parasites/cysts after 6- or 12-days post-switching to differentiation media. (C) Fluorescent images of DBA- or BAG1-positive Pru parasites/cysts after 12-days post-switching to differentiation media. (D) Fluorescent images of DBA-, BAG1-, SAG1-positive RH parasites/cysts after 2-, 7- and 12-days post-switching to differentiation media. (E) Fluorescent images of LDH-2p/GFP, SAG1/mCherry or DBA-positive parasites/cysts after 7- or 12-days post-switching to differentiation media. (F) Fluorescent images of Caco-2 and HFF infected with in vitro-differentiated bradyzoites at 72 hours post-infection. (G) Representative image of a brain cyst isolated from an infected mice fed with in vitro-generated bradyzoites after 28 days post-feeding. (PDF)