Changes in the number of BRD4 foci induced by the RNase III treatment in semi-permeabilized U2OS cells visualized by confocal microscopy.

<p><b>(A)</b> Representative figures show the localization of PIP2 and BRD4 using immunofluorescence staining. The last column shows the identified foci in false red color, which does not represent the intensity of the signal. Scale bars correspond to 5 μm. <b>B)</b> The chart visualizes the average number of BRD4 foci identified per cell in non-treated and RNase III treated semi-permeabilized U2OS cells. Statistical analysis was performed using Student’s t-tests (**** P < 0.0001), n = 4, N = 46 non-treated cells, N = 64 RNase III treated cells). Error bars correspond to SEM.</p> <p>(PDF)</p>