Deletion of <i>Rv3376</i>, <i>Rv3377c</i>, and <i>Rv3377c</i>-<i>Rv3378c</i>.

<p>Schematics of the gene replacement are shown for <i>Rv3376</i> (A), <i>Rv3377c</i> (C), and <i>Rv3377c</i>-<i>Rv3378c</i> (E). Validation by PCR amplification of the gene locus used primer sets flanking the target genes <i>Rv3376</i> (B), <i>Rv3377c</i> (D), and <i>Rv3377c</i>-<i>Rv3378c</i> (F). Corresponding primer sets are indicated as P1-P2 for <i>Rv3376</i>, P4-P5 for <i>Rv3377c</i>, and P4-P3 for <i>Rv3377c</i>-<i>Rv3378c</i> double mutant. (G) Growth curves of the Δ<i>Rv3378c</i>, Δ<i>Rv3378c</i>::<i>Rv3378c</i>, and Mtb H37Rv parent strains grown in 7H9 medium are shown. Uncropped gels for S1 Fig BDF and raw data for growth curves are provided in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.s013" target="_blank">S1 Data</a>.</p> <p>(PDF)</p>