Study on purification process and moisture absorption and moisture retention characteristics of Gleditsia sinensis seed polysaccharides

ObjectiveThis study aimed to optimize the purification process and evaluate the moisture absorption and retention properties of Gleditsia sinensis seed polysaccharides.MethodsThe adsorption and desorption performance of eight macroporous resins with varying polarities were compared using a static adsorption-analysis test to identify the optimal resin. Purification parameters for the selected resin were optimized based on adsorption and desorption efficiency. Moisture absorption and retention properties were evaluated under conditions of 43% and 81% relative humidity and in a dry silica gel environment, using glycerol, sodium alginate, and chitosan as references.ResultsD101 resin demonstrated the best purification performance. The optimal purification concentration of 2.00 mg/mL, a flow rate of 2 BV/h, a sample volume of 1.5 BV, an eluent concentration of 70%, and an eluent volume of 2 BV. Under the control of these conditions, the polysaccharide content increased from 51.90% to 82.40%. The moisture absorption rates of the polysaccharides were （20.35±0.006）% and （36.56±0.005）% at 43% and 81% relative humidity, respectively. The moisture retention rates were （84.66±0.014）% and （60.18±0.007）% under 43% relative humidity and dry silica gel conditions, respectively.ConclusionG. sinensis seed polysaccharides can be effectively purified using macroporous resin and exhibit excellent moisture absorption and retention properties, highlighting their potential for various applications.