Related to Fig 1.

<p>(A) A maximum intensity projection through the top portion of the distal region of one gonad arm from an L4 larva showing germ cell nuclei marked by HIS-11::mCH. The PZ is outlined, with an arrow indicating a crescent-shaped meiotic nucleus marking the proximal end of the PZ. Scale bar = 10 μm. The number of nuclei per PZ used to calculate the mitotic index (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011351#pgen.1011351.g001" target="_blank">Fig 1D</a>) is shown below. (B) A maximum intensity projection through the top portion of the distal region of the same gonad arm shown in (A) showing the GFP::TBB-2 signal. Arrow heads show examples of mitotic spindles in different stages of mitosis (prophase through telophase). The number of mitotic cells per PZ used to calculate the mitotic index (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011351#pgen.1011351.g001" target="_blank">Fig 1D</a>) is shown below. The dashed grey line indicates the median value for control. For all plots, dots represent one PZ and one PZ was assessed per animal. Boxplots show the median, interquartile range and most extreme values not considered statistical outliers. n.s. = <i>p</i> > 0.05, * = <i>p</i> < 0.05, ** = <i>p</i> < 0.01, *** = <i>p</i> < 0.001. Summary statistics and statistical tests used for all figure panels are given in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011351#pgen.1011351.s010" target="_blank">S2 Data</a>.</p> <p>(PDF)</p>