Optimization of liquid fermentation conditions of pectinase-producing Paenibacillus sp. C21 and its application in biological degumming of ramie fiber

The aim of this study was to develop the optimal liquid fermentation technology of pectinase produced by Paenibacillus sp. C21 and promote the application of biological degumming technology in ramie. Based on single factor tests, the fermentation conditions of pectinase-producing bacterium C21 were optimized by orthogonal tests, and the ramie was degummed by microorganism under the optimal culture conditions. The results showed that the optimal fermentation conditions of strain C21 for pectinase production were glucose 8 g/L, peptone 5 g/L, inoculum 3%, initial pH 9, fermentation temperature 32 ℃ and time 36 h. Under these optimal conditions, the pectinase activity could reach 206.37 U/ml. The microbial degumming test of ramie was carried out under the optimal fermentation conditions, and the weight loss of ramie fiber after treatment was 30.4%, which showed that the strain C21 had a good degumming effect on ramie and had the potential to be applied to ramie degumming industry.