<i>In Vivo</i> Injection of Reversible Optically\nControlled Short Interfering RNA into Japanese Medaka Embryos (Oryzias latipes) to Regulate Gene Silencing

Photoswitchable <i>ortho</i>-functionalized\ntetrafluorinated\nazobenzene-modified siRNAs (F-azo-siRNAs) were synthesized using solid-phase\nphosphoramidite chemistry. The activity of an F-azo-siRNA targeting\nenhanced green fluorescence protein (eGFP) in transgenic (Tg) Japanese\nMedaka (<i>Oryzias latipes</i>) was reversibly photocontrolled\nwith blue (470 nm) and green (530 nm) light, to activate and inactivate\nthe siRNA, respectively. This study highlights the first reversible <i>in vivo</i> study with photoswitchable siRNA. Controlling siRNA\nfunction reversibly <i>in vivo</i> could open new opportunities\nfor biotech research to better understand gene function and cellular\nmechanisms.