Electrochemical\nProfiling of vWFA2 for Systemic Inflammatory\nState Detection

This research aims to develop a portable biosensor device\nfor quickly\ndetecting vWFA2, a biomarker for inflammatory conditions. This sensor\ncould dramatically change detection methods and lead us to improve\nthe sensitivity of our tests to overcome the limitations of conventional\ndetection methods. Our label-free biomolecular assay is constructed\non an Au-ZnO electrode surface and uses electrochemical impedance\nspectroscopy (EIS) to measure the capacitive change in impedance,\nrevealing the binding effects of the target vWFA2, to the capture\nprobe. Our developed biosensor platform exhibits greater sensitivity\nand specificity, covering a wide dynamic range of 750–24,000\npg/mL and showing a strong correlation with inflammatory conditions.\nThis sensor exhibited a greater accuracy ranging from 86–110%\nfor the known spiked concentrations in nondiluted or modified plasma\nsamples. This electrochemical sensor has the potential to advance\npoint-of-care diagnostic methods due to its high sensitivity and rapid\nresponse time. The vision behind this research is to develop an electrochemical\nsensor that can rapidly and accurately detect disease states, thus\ncreating a pivotal prognostic tool in inflammatory state treatment\nand ultimately mitigating severe mortality and morbidity.