PAPreC: A Pipelinefor Antigenicity Prediction ComparisonMethods across Bacteria

Antigenicity prediction plays a crucial role in vaccine development, antibody-based therapies, and diagnostic assays, as this predictive approach helps assess the potential of molecular structures to induce and recruit immune cells and drive antibody production. Several existing prediction methods, which target complete proteins and epitopes identified through reverse vaccinology, face limitations regarding input data constraints, feature extraction strategies, and insufficient flexibility for model evaluation and interpretation. This work presents PAPreC (Pipeline for Antigenicity Prediction Comparison), an open-source, versatile workflow (available at https://github.com/YasCoMa/paprec_nx_workflow) designed to address these challenges. PAPreC systematically examines three key factors: the selection of training data sets, feature extraction methods (including physicochemical descriptors and ESM-2 encoder-derived embeddings), and diverse classifiers. It provides automated model evaluation, interpretability through SHapley Additive exPlanations (SHAP) analysis, and applicability domain assessments, enabling researchers to identify optimal configurations for their specific data sets. Applying PAPreC to IEDB data as a reference, we demonstrate its effectiveness across the ESKAPE pathogen group. A case study involving <i>Pseudomonas aeruginosa</i> and Staphylococcus aureus shows that specific feature configurations are more suitable for different sequence types, and that ESM-2 embeddings enhance model performance. Moreover, our results indicate that separate models for Gram-positive and Gram-negative bacteria are not required. PAPreC offers a comprehensive, adaptable, and robust framework to streamline and improve antigenicity prediction for diverse bacterial data sets.