Fe<sup>0</sup>‑MAP\nPrepared Glycosurfaces for\nSelective Cell Capture: From Adherent to Suspended Cells

The specific capture of live cells is crucial for various\nbiomedical\napplications. Existing methods often are limited by complex production\nprocesses. This study introduces Fe<sup>0</sup>-mediated monomer-adaptation\npolymerization (Fe<sup>0</sup>-MAP), a convenient and rapid synthesis\napproach for selective cell capture using surface-engineered glycopolymer\nbrushes. This method utilizes surface-initiated zerovalent iron-mediated\nreversible-deactivation radical polymerization (Fe<sup>0</sup>-SI-RDRP),\noffering advantages like simplicity, biocompatibility and oxygen-tolerance\ndue to the use of iron sheet as catalysts. We successfully employed\nFe<sup>0</sup>-MAP to selective capture both adherent (HeLa, L929)\nand suspended cells (Ramos, U937) in mammalian cell cultures. Combining\nexcellent biocompatibility, specific and reusable cell capture capabilities,\nand applicability to suspended cells, Fe<sup>0</sup>-MAP establishes\nitself as a promising strategy for selective cell capture, holding\nsignificant potential for diverse biomedical applications.