Nondestructively\nAssemble Cell Membrane-Coated Nanoparticles\nby Host–Guest Interactions for Efficient Capture of Bioactive\nCompounds

Cell membrane-coated nanoparticles (CNPs) have emerged\nas an attractive\nnanomedical tool. The basic premise is that the surface properties\nof natural cells can be integrated with the physical and chemical\nproperties of nanoparticles by coating them with cell membranes. However,\nthe degree of preservation of membrane proteins on nanoparticles,\na key indicator related to the biomedical function of these biomimetic\nsystems, is largely affected by the coating process. Herein, we report\na supramolecular cell membrane conjugation strategy mediated by host–guest\ninteractions to assemble CNPs without compromising protein activities.\nβ-cyclodextrin (β-CD) was rapidly and stably inserted\ninto the cell membrane by a lipid anchor without affecting the function\nof membrane proteins, thus attaching host–guest sites to the\nmembrane surface. By harnessing the excellent binding affinity between\nβ-CD attached to the membrane surface and adamantane, a supramolecular\ncell membrane-magnetic nanoparticle conjugate (CDM@AMNPs) was synthesized.\nThanks to the nondestructive assembly of this strategy, CDM@AMNPs\nwere endowed with a greater number of active binding sites, exhibiting\nefficient adsorption performance. This supramolecular conjugation\nstrategy mediated by nonreceptor site-based host–guest interactions\nproposes a scalable and cell-friendly strategy for the development\nof highly efficient CNPs.