The Addition\nof Transcriptomics to the Bead-Enabled\nAccelerated Monophasic Multi-Omics Method: A Step toward Universal\nSample Preparation

Omics-based measurements enable the study of biomolecules\nin a\nhigh-throughput fashion, leading to the characterization and quantification\nof biological systems. Multi-omics methods aim to incorporate several\nomics measurements for a more holistic approach, which is crucial\nfor advancing our understanding of the diversity and redundancy of\nbiological systems. Current multi-omics sample preparation methods\nhave achieved proteomics, lipidomics, and metabolomics from individual\nsamples; however, the bioinformatic tools currently available for\ninterpreting data generated from these omics are limited. Alternately,\ntranscriptomics has a wide arsenal of available bioinformatic tools\noffering intensive sample characterization but has yet to be incorporated\ninto a unified, multi-omics sample preparation technique. Herein we\ndescribe the modified bead-enabled accelerated monophasic multi-omics\n(mBAMM) method, which incorporates RNA extraction for transcriptomics\nanalysis. mBAMM was shown to enable RNA-seq without compromising the\nisolation of biomolecules for proteomics, lipidomics, and metabolomics.\nThis methodology greatly improves sample characterization and represents\na major innovation toward cohesive insights into biological systems.