Fluorescent Thermometers Based on Carbon Quantum Dotswith Various Detection Modes for Intracellular Temperature Measurement

We report that carbon quantum dots (CQDs) synthesized via a hydrothermal process using anthraquinone derivatives and l-cysteine provide versatile detection modes, making them suitable for various experimental setups. By modification of the precursor structures, these CQDs can function as different types of fluorescent nanothermometers, including those based on fluorescence intensity, ratiometrics, and fluorescence lifetime. Notably, fluorescence lifetime-based CQDs demonstrate robust performance under a wide range of conditions, including variations in pH and ionic strength. The CQDs exhibit low cytotoxicity and high cellular uptake efficiency, enabling wash-free imaging and precise fluorescence lifetime-based temperature measurements at the single-cell level. Furthermore, we successfully measured temperature changes associated with biochemical reactions, including the increase in cellular temperature induced by mitochondrial depolarization. In addition, these fluorescence lifetime-based measurements could be cross-verified using their fluorescence intensity. These findings underscore the potential of CQDs as versatile and minimally invasive tools for nanoscale thermometry in live cells.