Utilization of

Currently, various types of feed additives are being explored as alternatives to growth-promoting antibiotics, and larvae juice is emerging as a promising candidate. This study aimed to evaluate the potential of microorganisms contained in larvae juice as probiotics and determine the optimal dose for male broiler chickens. A total of 250 male Cobb broiler chickens were randomly divided into five treatments with five replications, each containing 10 chickens placed in separate cages. The treatments given included: positive control (PC), negative control (NC), larvae juice 1 mL/L (LJ1), larvae juice 3 mL/L (LJ3), larvae juice 5 mL/L (LJ5). Analysis revealed the predominance of gram-positive bacteria, such as Bacillus and Actinomyces, in the larvae juice probiotics. Giving probiotic larvae juice at a dose of 5 mL/L in drinking water significantly increases body weight and average daily gain, thereby providing a positive effect on saving feed costs. In conclusion dosage 5 mL/L are considered safe for broilers and do not adversely affect their physiological vital organs, immune system, digestive organs, or blood profile.