Black mulberry (Morus Nigra) callus tissue lysate effect on mouse blood leukocytes

The purpose of the research was to study in vitro the effect of black mulberry (Morus nigra) callus tissue lysate (MNCCL) on the ability of peripheral blood leukocytes of experimental animals to migrate from a glass capillary. The results were expressed as leukocyte migration index (LMI). It was found that in vitro in the presence of MNCCL the value of LMI ranges from 63 to 125. That is, both inhibition and stimulation of leukocyte migration are observed. When esophageal tissue antigen (ETAG) is introduced into the incubation medium, the value of LMI also fluctuates from a sharp inhibition of migration to significant stimulation. The aim of the study was to investigate in vitro the effect of a black mulberry (Morus nigra) callus cells lysate (CCL) on the ability of peripheral blood leukocytes of experimental animals to migrate from a glass capillary. The effect of CCL on the functional activity of mouse blood leukocytes (MBL) was studied in a leucocytes migration inhibition reaction (LMIR) in a modification of one of the authors of the article. The results were expressed as a leukocyte migration index (LMI). It was found that in vitro, CCL significantly affects the migration of MBL from a glass capillary. LMI ranged from 63 to 125. That is, from inhibition to pronounced stimulation of migration. Moreover, when a tissue antigen of the esophagus (ETA) is introduced into the incubation medium, CCL modulates not only the spontaneous but also the tissue antigen-induced migration of leukocytes. In this case, the value of LMI also ranged from a sharp inhibition of migration to significant stimulation. That is, CCL in vitro exhibits the properties of both pro- and anti-inflammatory cytokines.