Alternative Method\nfor Glyphosate Determination in\nUnroasted Green Coffee Beans by Liquid Chromatography Tandem Mass\nSpectrometry (LC–MS/MS)

This research proposes an alternative method to detect\nand quantify\nglyphosate residues in unroasted green coffee beans by LC–MS/MS.\nThe sample preparation was conducted without derivatization steps,\nwith integrated cleanup, which improves the analytical method’s\nfrequency. Validation results were consistent with the requirements\nof the regulatory guidelines employed. Specificity, linearity (<i>r</i><sup>2</sup> = 0.9991), precision (RSD ≤ 9%), and\nrecovery (92–112%) were ensured, with a satisfactory limit\nof quantification (LOQ = 0.48 mg kg<sup>–1</sup>). These data\ndemonstrate that the method is suitable for monitoring glyphosate\nresidues in unroasted coffee beans while offering simplicity and speed\nin sample preparation. The method was applied to analyze authentic\nunroasted coffee bean samples, in which two of them were contaminated\nwith glyphosate (