Bioorthogonal\nSynthesis of Biomimetic Nanoparticles\nfor Screening Chemical Hazards in Food Samples

The ability to identify unknown risks is the key to improving\nthe\nlevel of food safety. However, the conventional nontargeted screening\nmethods for new contaminant identification and risk assessment remain\ndifficult work. Herein, a toxic-oriented screening platform based\non high-expression epidermal growth factor receptor HEK293 cell membrane-coated\nmagnetic nanoparticles (EGFR/MNPs) was first used for the discovery\nof unknown contaminants from food samples. The EGFR served as a bait\nto bind the risk compounds, considering that the abnormal activation\nof EGFR was related to the incidence of numerous chronic inflammatory\ndiseases. The cell membranes were specifically covalently immobilized\non magnetic carriers through metabolic glycoengineering and strain-promoted\nalkyne–azide cycloaddition (SPAAC) to ensure the stability\nand bioactivity of the EGFR/MNPs. The synthesized EGFR/MNPs possessed\nan excellent receptor loading amount and were used to screen potential\nrisk substances from thermally processed food. Finally, two compounds,\nharman and norharman, were rapidly identified. The toxicological experiments\nconfirmed that the screened compounds could promote EGFR phosphorylation\nand further activate downstream signaling pathways, thereby inducing\ncellular oxidative damage. The proposed method provided a toxic-oriented\nmethod for identifying risk compounds from food samples.