Janus Separation-Sensing\nMembrane Hosted with Enzyme@MOF\nNanoreactor for Real-Time Blood Sensing

Plasma separation, rich in biomarkers crucial for diagnosis,\nis\nconventionally achieved via high-speed centrifugation, a method hindered\nby its blood usage, lengthy processes, and complex operations, which\ndelays detection. We introduced a novel real-time blood sensing method\nbased on a Janus membrane and enzymes @MOFs. Asymmetric driving of\nthe janus membrane can realize spontaneous separation of plasma and\nprevent hemolysis during direct separation. Glucose oxidase (GOx),\nuric acid oxidase (UOx) and horseradish peroxidase (HRP) were encapsulated\nin a hydrophilic organometallic framework (MOFs) to construct an enzyme\ncascade nanoreactor. Embedding enzyme in hydrophilic MOFs not only\nretains the natural conformation of free enzyme but also improves\nthe brittleness of enzyme, endows MOFs with new biological functions,\nand expands its sensing application. Using 3,3′,5,5′-tetramethylbenzidine\n(TMB) as a chromogen and a custom app for color interpretation, we\nachieved real-time visualization of glucose (Glu) and uric acid (UA)\nat a 50 μM limit. The system accurately analyzed serum samples,\nmatching commercial kits and showing promise for portable, personalized\ndiagnostics.