Sperm Motility of Cryopreserved Buffalo Semen After Thawing at Different Temperatures and Duration

This study investigates the effect of different thawing temperatures and durations on the motility ofspermatozoa in cryopreserved buffalo semen. A total of 40 buffalo semen straws from the LembangArtificial Insemination Center (batch code AW 1205) were used. The experimental design was acompletely randomized design with 10 replications and 4 treatment groups: P0 (37°C for 30 seconds), P1(28°C for 30 seconds), P2 (28°C for 45 seconds), and P3 (28°C for 60 seconds). Sperm motility wasevaluated using a microscope at 400x magnification, and motility was based on the percentage of spermmoving progressively. Statistical analysis was performed using ANOVA followed by Duncan's MultipleRange Test. The results showed that the highest motility (43.50%) was observed in P3 (28°C for 60seconds), followed by P0 (43%), P2 (35.50%), and P1 (31.59%). These findings suggest that thawingbuffalo semen at 28°C for 60 seconds provides the highest sperm motility, which could improve theefficiency of artificial insemination programs.