A Phytotherapeutic Approach to Wound Healing: Evaluation of a Dual-Herb Gel Containing Euphorbia hirta and Tridax procumbens

This study provides an emphasis on the formulation and evaluation of a polyherbal wound healing gel from Euphorbia hirta and Tridax procumbens. The plants are known to have been traditionally used in treating wounds and skin disorders due to their rich phytochemical profile. All tests were performed on gelling properties such as colour, appearance, pH, spreadability, homogeneity, viscosity, stability, and antibacterial activity. From among three developed formulations and completed with the total weight of three batches in which F3 showed the best features, which were a stable pH value of 6.9 and also showed an excellent spreadability with a batch free from microbial contamination.This present work shows a study on formulation and evaluation of a polyherbal wound healing gel with Euphorbia hirta and Tridax procumbens. Both plants are known for their traditional usefulness against wounds and skin disorders due to the high purport of several different phytochemical profile. The prepared gels were evaluated on parameters like colour, appearance, pH, spreadability, homogeneity, viscosity, stability, and antimicrobial activity. Out of the three formulations developed, batch F3 showed optimum characteristics, while stable pH with excellent spreadability made it free from microbial contamination. Phytochemical analysis corroborated the presence of bioactive constituents like flavonoids, alkaloids, tannins, and saponins. Molecular docking studies provided further support for wound healing potential by showing the strong interactions of Gallotannins and Quercetin with the active site residues of several key wound-related protein targets. These findings suggest that the formulated polyherbal gel can be deemed safe, stable, and efficacious for topical wound healing applications, thus paving the way for its further development for herbal therapeutics.