GRASP: a modular toolkit for building synthetic pentatricopeptide repeat RNA-binding proteins

GRASP Repository for the "Golden Gate Assembly for Synthetic PPRs" kit Data onein20.csv — proportion of edited reads for each RNA variant in each well of the plate in the assay pvariants.tsv — metadata for each protein variant rvatiants.tsv — metadata for each RNA variant SS_motifs.tsv — Sequences of 31 aa S motifs (SS motifs as defined in Cheng et al. 2016) with standard fifth-last combinations (TN, NN, TD, ND) extracted from the 1KP dataset reported in Gutmann et al. 2020 composition/selaginella/.CDS.fasta — edited sequences of Selaginella organellar coding sequences composition/selaginella/.targets.csv — extracted sequences of putative PPR binding sites (-4 to -15 regions of editing sites) composition/angiosperms/.CDS.fasta — edited sequences of angiosperm organellar coding sequences composition/angiosperms/_targets.csv — extracted sequences of putative PPR binding sites (-4 to -15 regions of editing sites) Code Figs4_5A_6A.ipynb — Jupyter notebook in Julia to generate the plots for Figs 4, 5A, 6A Figs6B_6C.ipynb — Jupyter notebook in Julia to generate the plots for Figs 6B, 6C composition/selaginella/Selaginella_target_composition.ipynb — Jupyter notebook in Julia to generate the Selaginella panel for Fig 5B composition/angiosperms/Angiosperm_target_composition.ipynb — Jupyter notebook in Julia to generate the Angiosperm panel for Fig 5B Figures *.svg — generated figures in Scalable Vector Graphics (SVG) format