Alternative procedure for obtaining nanofat: analysis, culture, and clinical application

Background: The mechanical extraction of body fat can alter the structure of adipose tissue, and its primary effect on transfer to other sites may be affected. In addition, it appears to be related to the activity of mesenchymal stem cells. Since its introduction, nanofat has been used for various regenerative treatments, such as scar improvement, aesthetic treatments, burns, irradiated tissue, and chronic wounds. Objective: The main objective of this study is to present an alternative procedure for obtaining nanofat, its viability analysis, culture, and clinical application. Materials and methods: A novel procedure for obtaining nanofat was developed. Each of the steps of the procedure is described, and the microscopic analysis of the mesh used and histology of the nanofat samples were also performed. In addition, mesenchymal stem cell derivation and immunophenotypic identification and their clinical application were carried out. Results: The proposed procedure allowed obtaining nanofat in a closed manner, systematizing each step. Many mesenchymal stem cells with adequate results in their clinical application were identified. The study found that nanofat therapy significantly improved skin texture and radiance. Conclusions: The proposed procedure is an alternative for obtaining nanofat and mesenchymal stem cells for their clinical application and in research studies in tissue engineering and regenerative medicine therapies. Keywords: Nano, adipose tissue, mesenchymal stem cells.