Analytical Method Development and Validation of Chlorpheniramine Maleate by Uv Spectroscopy

A UV Spectrophotometric method for the determination of Chlorpheniramine Maleate in Bulk and its pharmaceutical dosage form. The wavelength was selected using water, 0.1 N HCl and 0.1 N NaOH as solvents (later HCL was selected as final solvent). The method was found to be specific with the appearance of the corresponding maxima at 268 nm. Linearity was observed at concentration of 10µg/ml – 50µg/ml and a correlation coefficient (R2) of 0.997. Limits of detection and quantification were respectively 0.62 and 0.187. The tolerance limits for the different concentration levels (80, 100 and 120%) were respectively: 100.2, 100.5 and 99.5%. The results of method validation were in the acceptable range as per ICH guidelines