Nanoliposomes Containing Iodine and ZIF-8 Conjugated with AntiCD20 Antibody: One nanoplatform for treatment and imaging

Abstract: Multifunctional nanoparticles have used for the concurrent determination and treatment intense and incessant fiery maladies. Our aim was to plan, synthesize and characterize nano-liposomes containing iodine and ZIF-8 conjugated with anti-CD20 antibody and to examine Its differentiate property against X-rays. Their biocompatibility and antiinflammatory properties of this nanoplatform was investigated invitro. In this study, the synthesis and characterization of nano-liposomes containing iodine and ZIF-8 conjugated with anti-CD20 antibody were performed using methods such as TEM, SEM, FTIR and DLS. The HU (Hansfeld) number of the synthesized nano-liposomes was determined using CTscan. Then, the cell death of white blood cells after exposure to synthesized nanoliposomes was measured by MTT test. Also, the anti-inflammatory properties of synthesized nanoliposomes were evaluated by ELISA kit measuring the amount of interferon gamma. Microscopic imaging with TEM and SEM and analysis with DLS showed that nanoliposomes containing iodine and ZIF-8 and conjugated with anti-CD20 antibody were spherical and had a size range of 150 to 300 nm. The binding of antibody on the surface of nanoliposomes was also confirmed in FTIR test. This study showed that the percentage of iodine loading was 76% and ZIF8 was 72% in synthesized nanoliposomes. Also, the percentage of antibody conjugation on synthesized nanoliposomes was reported to be 86%. Imaging with a CTscan device also showed that there was no significant difference between the Hounsfield number of different nanoliposomes. Also, the concentration dependence was not observed. MTT test also showed that among the different substances tested, iodine and ZIF8 nanoparticles had significant toxicity compared to the other substances tested at all concentrations. This shows that nanoliposomization of iodine and ZIF8 reduced toxicity on white blood cells. Also, ELISA test and determination of the amount of interferon gamma secretion clearly showed that anti-CD20 antibody and nanoliposomes containing this antibody were able to significantly reduce interferon gamma secretion by human white blood cells at all concentrations studied.