Anti –Oxidant Activity of Methanolic Extract of Azadirachta Indica Flowers

Azadirachta indica, commonly known as Neem, is a fast-growing, evergreen tree indigenous to India. All parts of the plant, including leaves, bark, seeds, roots, and flowers, are widely used in traditional medicine [1]. This study investigates the antioxidant potential of Neem flowers using a hydrogen peroxide (H₂O₂) scavenging assay. Extraction was collected by maceration, using the Methanol. Phytochemical screening was done by following schematic procedure and the Antioxidant activity was assessed based on the H2O2 (Hydrogen Peroxide) Free Radical Scavenging Activity Assay method, involving spectrophotometric measurement at 230 nm. The extract demonstrated the highest antioxidant activity, reaching 182.34% at 100 μg /ml, with an IC₅₀ value of 67 μg /ml, compared to the ascorbic acid standard with an IC₅₀ of 78.84 μg/ml. [5] Phytochemical screening confirmed the presence of flavonoids, phenols, and carbohydrates. The study reinforces the growing interest in plant-based therapies due to their safety, affordability, and minimal side effects when compared to conventional synthetic drugs. [2]