Development and Evaluation of Herbal Formulation (Face Serum) By Using Piper Betle Leaf Extract

In the Indian system of medicine-Ayurveda, piper betle has been mentioned as a remedy for treatment of various infectious diseases and ailments. Based on the folkloric use, the present study was designed to formulate and evaluate polyherbal serum containing extracts of Piper betle. Serum formulations (Formulation 1, 2 and 3) were prepared which comprised of the methanolic extracts of Piper betle in a concentration of 100mg, 200mg and 300mg, respectively in a base. The prepared formulations were evaluated for appearance, pH, homogeneity, viscosity, spreadability, skin irritation test (patch test) and stability. The formulations were also screened for their antimicrobial activity by well diffusion method against S. aureus, and E. coli. The results of the studies revealed that all formulations under study viz. A, B and C showed better zone of inhibition as compared with the control. However, formulation C exhibited maximum activity against the selected strains which may be attributed to its greater amount of herbal extracts as compared to formulation A and B. The polyherbal gel formulations were observed to possess antimicrobial action. The effective activity may be attributed to the synergistic action of the plants constituents present in the formulation.