Formulation And Evaluation of Ophthalmic in Situ Gel for Treatment of Fungal Keratitis

The objective of this research was to formulate and evaluate the latent use of the in situ gel preparations for ocular delivery of posaconazole for the treatment of fungal keratitis. An in situ gelling system was used to rise the residence time and thus the bioavailability of posaconazole in ocular mucosa. In situ gel preparations were formulated by cold method using polymers like poloxamer 407, poloxamer 188 and sodium alginate. Finally, concentration of posaconazole in formulations was 0.2% (w/w). These formulations were evaluated for pH, solution-gel transition temperature, gelling capacity, drug content, viscosity and clarity. Gelation temperature of each and every one of the preparations was within the range of 32-34ºc. All the preparations exhibited fairly constant drug content. Also in-vitro drug release and anti-fungal action of these preparations were also estimated. Drug release study of all the preparations exhibited sustained release properties. In conclusion, posaconazole loaded in-situ gels could be presented as a promising approach for optical drug delivery for the behavior of fungal diseases.