Isolation and Screening of Invertase Producing Fungi from Textile Sizing Site

Abstract Filamentous fungi are important due to their high enzyme production potential. Many enzymes produced by fungi have been related to biotechnological applications in several industrial sectors. This research explores the isolation and characterization of invertase-producing fungi from textile sizing sites, with a focus on their biotechnological potential. Invertase (β-fructofuranosidase) is an enzyme that catalyses the hydrolysis of sucrose into glucose and fructose, making it invaluable in various industrial applications, including the food and beverage sectors. Soil samples were systematically collected from multiple textile sizing locations, and fungal strains were isolated using selective media. Total ninety fungi were isolated and screened for invertase production potential on Czepak Dox medium. Invertase producing colonies were detected by the formation of orange to reddish precipitate around the growth of the fungi. Out of ninety fungal isolates thirty-seven fungal colonies were found to be positive for invertase production. The use of enzyme technology to meet various human needs is becoming more popular. This study not only enhances the understanding of microbial diversity in waste environments but also highlights the feasibility of utilizing these organisms for sustainable enzyme production, thereby contributing to advancements in bioprocessing and environmental bioremediation