Antibody Characterization Report for Slo3

This article presents antibody characterization data that can be utilized as a guide to select high-performing antibodies for Slo3, encoded by the KCNU1 gene. Five commercial human or mouse reactive antibodies were evaluated by western blot following a standardized protocol which compares read-outs in isogenic parental controls and knockout cell lines. The antibodies were tested by western blot on WT and KCNU1 KO testis/epididiymis tissues from mouse to recognize mouse Slo3 as well as lysates from a HEK293 KCNU1 overexprssion cell line to recognize human Slo3. Thank you to Dr. Celia Santi at Washington University School of Medicine, USA for donating the mouse tissues. We'd also like to thank the Bill and Melinda Gates Foundation for their support and nomination of this target as well as the Structural Genomics Consortium for coordinating this study.