MIBIREM Deliverable 2.3 Metagenome and transcriptome of the six most promising microbiomes

This deliverable describes the sequencing of metagenomes and metatranscriptomes of the most promisingmicrobiomes with efficient degrading activities for the three use cases. These data (currently underacquisition) will be analysed to: i) reconstruct the genomes of each individual bacteria from the consortia(MAGs), ii) predict their biochemical pathways and potential role in the consortia, and iii) identify the genesand pathways that are over-expressed or repressed when the consortia are grown on contaminants andduring degradation. The metagenome and transcriptome were planned on the six most promisingmicrobiomes, but were actually performed on ten.This information will be used to select the best microbiomes for the next steps, and mainly for directedevolution. In addition, these data will be further used to target functional genes in bioaugmentation andbioremediation assays during pilot and field testing of our microbiomes.As a complement, ongoing actions will result in SIP-metabarcoding and metagenomic datasets that will beincluded in an updated version of the deliverable prior to M36.