Epigenetic Regulation of MIR-25 And LNC107153 On Expression of Seasonal Estrus Key Gene CHGA In Sheep

This study sheds light on the complex epigenetic regulatory processes that control the expression of chromogranin A (CHGA), a key gene that controls seasonal estrus in sheep. We show that microRNA-25 (miR-25) and long non-coding RNA 107153 (Lnc107153) control the expression of CHGA in a complex way through epigenetic changes using a wide range of methods, including bioinformatics analysis, quantitative real-time PCR, and functional assays. Our results show that miR-25 directly targets CHGA and lowers its expression. Lnc107153, on the other hand, functions as a competitive endogenous RNA by binding to miR-25 and changing CHGA expression. Our data also reveal that the miR-25/Lnc107153/CHGA axis is very important for controlling the seasonal reproductive cycle in sheep. This has big effects on how to improve breeding techniques and reproductive efficiency in small ruminants. This study gives us new information on the molecular processes that cause seasonal estrus, which could help us find new ways to improve sheep's reproductive performance.