The Effect of Chronic and Acute High-Intensity Training on the Macrophage (CD11b) Expressing NF-κB Due to Malondyaldehide (MDA) Level

Abstract Aimed: The purpose of this study was to determine the effect of chronic high-intensity eccentric training of 80%-90% VO2max and Acute high intensity eccentric training on the expression of CD11b and NFkB due to Malondyaldehide level. Methods: In this study twenty nine male mus musculus after one week of familiarization with environtment and training were randomly divided into three groups, control (n=10), chronic HIT (n=10) and acute HIT (n=9) were performed a dynamic high intensity training on animal treadmill where speed and endurance are done with a load of 80 - 90% of maximum capacity .The form of exercise used in this study is downhill treadmill training with a negative angle inclination of -15o for 23 minutes for 7 days on the chronic high intensity training group and one day for acute high intensity training group and then termination was carried out to collect the spleen of the mice which were then processed by flowcytometric method to observe the percentage of macrophage cells CD11b expressing NFkB. Results : The result of flowcytometry tests obtained that macrophage cells CD11b expressing NFkB showed that the mean of control group was 256.50+129.719, chronic high-intensity eccentric training group 251.10+149.637, acute high-intensity eccentric training group 273.78+101.867.Anova test significant level was 0.925 which mean that there were no significant difference between group. Conclusion: The mean of CD11b expressing NFkB of acute HIT group higher than control and chronic HIT group, while Compare Anova test didn’t showed significant difference between control, chronic and acute high intensity training group on macrophage cells (CD11b) expressing NFKB.