Effects of Trehalose and Tris-Aminomethane Egg Yolk Extenders on Semen Quality of Duroc Boars Across Cooling, Before Freezing, and Post-Thawing Conditions

This study aimed to evaluate the effectiveness of various semen extenders in preserving boar spermquality during cryopreservation, with a focus on the use of trehalose as a cryoprotectant. Semen sampleswere collected from a Duroc boar and subjected to three treatment groups: T0 (BTS extender), T1 (BTS +100 mM trehalose), and T2 (Tris-egg yolk extender). Semen was assessed at three stages: after cooling,before freezing, and post-thawing. The results showed that T1 significantly outperformed T0 and T2across all parameters—maintaining the highest sperm motility and viability, and the lowest abnormalitiesafter thawing. These findings indicate that trehalose effectively stabilizes sperm membranes and reducescryoinjury. Therefore, the use of trehalose-enriched BTS extender is recommended for improving postthaw semen quality in boars. Further research should explore field fertility outcomes and optimal trehaloseconcentrations.