Potential psychrotrophic bacteria isolated from Tawang, Arunachal Pradesh, India for use as biofertilizer

Nitrogen and phosphorus molecules, being an integral part of various physiological and biochemical processes, are indispensable for the growth and maturation of plant tissues. It is well-accepted that these molecules play a significant role in regulating agricultural productivity. This study aims to isolate and characterise Psychrotrophic microorganisms for use as biofertilizers using biochemical and molecular techniques. The main goal is to find microbial strains that can solubilize phosphorus and fix nitrogen under the harsh environmental conditions found in high-altitude areas. The research involved isolating bacteria soil samples collected from the Tawang region in Arunachal Pradesh and subjecting them to a comprehensive biochemical analysis to determine their phosphate solubilizing and nitrogen-fixing potential. The study discovered 54 bacterial isolates, 44 of which can solubilize phosphate and 10 strains capable of nitrogen fixation. Based on 16S rRNA gene sequence analysis, the isolates were found to belong to the genera Pseudomonas, Burkholderia, Azotobacter and Azospirillum.