A guide to selecting high-performing antibodies for Stearoyl-CoA desaturase (SCD1) (UniProt ID: O00767)

A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.160217.1 This report provides a guide for selecting high-quality commercial antibodies against Stearoyl-CoA desaturase (SCD1). Nine SCD1 antibodies were evaluated in western blot, immunoprecipitation and immunofluorescence. The characterization data was generated using a standardized protocol that compares read-outs in knockout cell lines and their isogenic parental controls. Thank you to the Work Michael J. Fox Foundation for Parkinson's Research for nominating this target and supporting the study.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2024-10-04
DOI
https://doi.org/10.5281/zenodo.13891494
Citations
2
Primary Topic
Fatty Acid Research and Health
Type
article
Field-Weighted Citation Impact
0.62
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article

A guide to selecting high-performing antibodies for Stearoyl-CoA desaturase (SCD1) (UniProt ID: O00767)

2 citations
Zenodo (CERN European Organization for Nuclear Research)
Fatty Acid Research and Health
0.62
article

A guide to selecting high-performing antibodies for Stearoyl-CoA desaturase (SCD1) (UniProt ID: O00767)

article en
2 citations

Abstract

A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.160217.1 This report provides a guide for selecting high-quality commercial antibodies against Stearoyl-CoA desaturase (SCD1). Nine SCD1 antibodies were evaluated in western blot, immunoprecipitation and immunofluorescence. The characterization data was generated using a standardized protocol that compares read-outs in knockout cell lines and their isogenic parental controls. Thank you to the Work Michael J. Fox Foundation for Parkinson's Research for nominating this target and supporting the study.

Zenodo (CERN European Organization for Nuclear Research)
Montreal Neurological Institute and Hospital (CA)
Openalex Percentile: Top 31%
Fatty Acid Research and Health
0.62
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A guide to selecting high-performing antibodies for Stearoyl-CoA desaturase (SCD1) (UniProt ID: O00767) · Zenodo (CERN European Organization for Nuclear Research) (2024) | TGRS Research Map | TGRS