VALUABLE Fermentation and yeast oil recovery processes

Draft deliverbale pending EC approval Cutaneotrichosporon oleaginosus is a highly promising oleaginous yeast for the industrial production of single-cell oil (SCO), demonstrating lipid accumulation of up to 85% of its dry cell weight. Its metabolic versatility allows the utilization of diverse carbon sources, while also exhibiting a high tolerance to inhibitory compounds. These characteristics make it a robust candidate for large-scale SCO production. The yeast fermentation process is designed to maximize intracellular lipid accumulation, followed by a downstream recovery process to extract SCO from the cells. This recovery step is critical to the overall efficiency of the production process, as it involves cell disruption and lipid separation from biomass and the aqueous phase of the fermentation medium, each of which affects the yield, purity, and economic viability of the final product.