Antioxidant and Antimicrobial Activities of Methanolic Extract of Sorghum Bicolor Seeds

The present study investigates the antioxidant and antimicrobial activities of the methanolic extract of Sorghum bicolor seeds. The extract was obtained using Soxhlet extraction with 80% methanol, yielding 68% of the extract. Phytochemical screening revealed the presence of cardiac glycosides, anthraquinone glycosides, and saponin glycosides. Antimicrobial activity was assessed against Bacillus megaterium, Bacillus thuringiensis, and Escherichia coli at concentrations of 20 mg/L, 100 mg/L, and 200 mg/L. Results showed dose-dependent antimicrobial activity, with the highest concentration exhibiting inhibition zones of 16 mm, 15 mm, and 18 mm for the respective organisms. The DPPH assay demonstrated moderate antioxidant activity, with a % inhibition of 47.33% at 1 mg/ml concentration, indicating significant antioxidant activity compared to the standard ascorbic acid (84.35%). These findings suggest that Sorghum bicolor seeds possess bioactive compounds with potential pharmacological applications, including antimicrobial and antioxidant properties.