Epidemiology, Etiology, Pathysiology, Physiology, Pharmacotherapy, Treatment and Diagnosis

Folic Acid is the water-soluble vitamin (vitamin B9) and lysine hydrochloride is Amino acid Supplement. Folic Acid and Lysine Hydrochloride used as Growth and Development of Children, folate deficiency, amino acid deficiency. Development and validation of simple, Precise and Accurate RP-HPLC method for simultaneous estimation of Folic Acid and Lysine in synthetic mixture. The validation of this method was achieved as per ICH Q2 (R2) guidelines with the optimized experimental conditions. To achieve the proposed method on C18 column (250 mm x 4.6 mm, 5 µm) column as Stationary Phase and run time was 30 min. The Mobile Phase consists of Methanol, Acetonitrile: Phosphate buffer (50:20:30). UV detection was carried out at 300nm. Linearity co-relation co-efficient found is Folic acid is 0.9993 and lysine hydrochloride is 0.9999. The method was validated by determining its accuracy, linearity and precision. The proposed method is simple, precise, economical and hence can be applied for routine quality control of Folic Acid and Lysine Hydrochloride in synthetic mixture.