Eutypa camelliae Samarakoon, M. C & Hyde, K. D

Eutypa camelliae Samarakoon, M. C & Hyde, K. D, Fungal Diversity 112 (1), 1–88 (2022) Fig. 2 Description. Saprobic on a dead branch of an unknown tree. Sexual morph: Stromata 3.1–8.3 mm diam., immersed in the bark, carbonaceous, effuse, confluent into irregularly elongated shape with diffuse margins, dark grayish to dull black surface, rarely with white dots on the surface, 20–50 loculate. Ascomata 390–620 µm in height, 190–340 µm in diameter (x ̄ = 530 × 260 µm, n = 10), perithecia, coated with a white powdery substance in between, vary from globose to akin to an inverted flask, Ostiole slightly raised, conspicuous, 114–128 μm wide. Peridium 13.5–25 μm thick, two-layered, outer layer dark brown of textural angular cell, inner layer hyaline of elongated cell. Paraphyses septate, 3.5–7.2 μm (x ̄ = 5.6 μm, n = 20) wide, constricted at the septa, longer than asci. Asci 50–120 × 4–6.6 μm (x ̄ = 77.5 × 5.4 μm, n = 30), 8 – spored clavate, with a rounded to truncate apex, J- apical rings. Ascospore 4–5.8 × 1.1–1.8 μm (x ̄ = 4.93 × 1.35 μm, n = 30), overlap, allantoid, slightly curved, subhyaline, smooth, aseptate, often with a guttulae at both ends. Asexual morph: undetermined. Culture characteristics. Colonies on PDA reach 60 mm in diameter after seven days at 28 ° C. They are cottony, moderately dense, fluffy aerial mycelium, white from above and pale yellowish from below. Mycelium is composed of branched, septate, smooth-walled, hyaline hyphae. Specimens examined. China • Guizhou Province, Kaili City, Leigongshan State Reserve (108°11'47"E, 26°22'43"N), altitude 1664 m, on a dead branch of an unknown tree, 23 August 2023, Xin Y Mao & Y. Q. Kang, LGS 19 (GZAAS 24-0012, KUN-HKAS 133146, strain number GZCC 24-0187). GenBank accession numbers (ITS: PP 528179; tub 2: PQ 301429). Guizhou Province, Libo County, MaoLan National Nature Reserve (108°4'9"E, 25°17'8"N), altitude 694 m, on dead branches of an unknown tree, 22 March 2022, Xin Y Mao & Y. Q. Kang, LBML 10 (GZAAS 24-0013, KUN-HKAS 133147; strain number GZCC 24-0188). GenBank accession numbers (ITS: PP 528182; tub 2: PQ 301430). Notes. The sequence of our collection GZAAS 24-0012 clustered with Eutypa camelliae in the phylogenetic tree, and ITS sequence BLAST searches also confirmed a 100 % match with E. camelliae (HKAS 107022). The holotype description of this species was based on immature stromata, no asci or ascospores were observed in the material and the isolates were obtained from internal tissue of the stromata (Samarakoon et al. 2022). Our study represents the first report of Eutypa camelliae from China and provides the complete anatomical details, including mature stromata with asci and ascospores.