In Vitro Anti-Inflammatory, Antiplatelet, And Antioxidant Activities of Cassia Fistula Linn Leaves

Cassia fistula, commonly known as "Golden Shower," is a medicinal plant with a wide range of traditional uses. This study aimed to investigate the in vitro anti-inflammatory, antiplatelet, and antioxidant activities of Cassia fistula Linn. leaves. The leaves of Cassia fistula were extracted using an appropriate solvent, and the extract was subjected to in vitro assays to evaluate its potential therapeutic properties. The anti-inflammatory activity was assessed by measuring the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. The antiplatelet activity was determined by evaluating the inhibition of platelet aggregation induced by adenosine diphosphate (ADP) and collagen. The antioxidant activity was evaluated through assays measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and total antioxidant capacity. The results demonstrated that Cassia fistula leaf extract exhibited significant in vitro anti-inflammatory activity by inhibiting NO production in LPS-induced macrophages. The extract also displayed potent antiplatelet activity by inhibiting platelet aggregation induced by ADP and collagen. Additionally, the extract exhibited remarkable antioxidant activity by effectively scavenging DPPH radicals and demonstrating high total antioxidant capacity. These findings suggest that Cassia fistula leaf extract possesses notable in vitro anti-inflammatory, antiplatelet, and antioxidant activities. These pharmacological properties may contribute to the plant's traditional medicinal uses and highlight its potential as a valuable natural resource for the development of therapeutic interventions. Further investigations are required to identify and isolate the bioactive compounds responsible for the observed activities and to explore their mechanisms of action.