Evaluation of Antibacterial Activity of Crude Extracts of Nyctanthes Arbor-Tristis Leaves Against Staphylococcus Aureus, Escherichia Coli, Pseudomonazs Aeruginosa and Bccillus Subtilis

The management of various diseases and the age-old subject of treatment along with Ayurveda and home remedies play a crucial role in the Human therapy. Therefore, adopting an idea from home remedies and Ayurveda, these studies was designed and conducted to demonstrate the anti-bacterial application of Nyctanthes arbor-tristis leaves scientifically. Solvents acetone, ethanol, acetic acid, and water were utilized to prepare the extracts.Antibacterial activities of various extracts were performed against four bacteria, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bccillus subtilis, by agar cup diffusion technique. Zone of inhibition caused by various extracts were quantified. Acetic acid extract of Leaves of Nyctanthes arbor tristis exhibited antibacterial activity against all tested bacteria in the sequence of sensitivity as Staphylococcus aureus>Pseudomonas aeruginosa>Escherichia, coli>Bacillus subtilis. Antibacterial effect of Staphylococcus aureus was sensitive having 8, 12, 24 and 6 mm zone of inhibition at 10 mg mL-1 of Acetone, Ethanol, Acetic acid and Water extracts respectively. Zone of inhibition is 10, 12, 20, 8 mm for Acetone, Ethanol, Acetic acid and Water extracts respectively for, Pseudomonas aeruginosa. Escherichia coli demonstrated no zone of inhibition for water extract while it has 2, 10, 18 mm zones of inhibition at 10 mg mL-1 for Acetone, Ethanol, Acetic acid respectively. Bacillus subtilis exhibited 4, 9, 20 and 3 mm zone of inhibition at 10 mg mL-1 for Acetone, Ethanol, Acetic acid and Water extracts. The leaves of the plant are observed to act as the highest green constituent with anti-bacterial activity against various Staphylococcus Aureus, Escherichia Coli, Pseudomonazs Aeruginosa and Bccillus Subtilis pathogens studied.These findings indicated that the Acetic acid extract possesses a broad spectrum of antibacterial activity compared to the Acetone, Ethanol and Water extracts.