A Review On Analytical Method Development And Validation For Broccoli

A simple accurate and selective methods are developed and validation for determination of phytoconstituents in broccoli by products. Broccoli is a fresh green vegetable in the cabbage family and it contains main phytoconstituent is sulforaphane which is found in isothiocyanate group of organosulfur compounds. The sulforaphane is mainly used to prevent cancer. In this review, the methods were developed and it also includes validation parameters for the determination of phytoconstituents in broccoli. Results are consistent with expected ranges. The broccoli UV range results in three bands UVA 315-400 nm, UVB 280-315 nm, UVC 100-280 nm. The detection limits and quantitative limits of these compounds were in the range of 0.15-0.46 and 0.42-2.47 µg/ml respectively.